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Avian Influenza Virus H5 (AIV H5) Antigen Rapid Test Cassette

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Avian Influenza Virus H5 (AIV H5) Antigen Rapid Test Cassette

Brand Name : VECHEK

Model Number : VIAIH-502

Format : Cassette

Packaging : 10T

Product Name : Veterinary Rapid Test Kits

Sample Type : Laryngotracheal secretion/Feces

Shelf Life : 24 months

Storage Temperature : 2-30℃

Test Method : Immunochromatographic Assay

Test Time : 15 minutes

Test Type : Rapid Test

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Avian Influenza Virus H5 (AIV H5) Antigen
Rapid Test Cassette
(Laryngotracheal secretion/Feces)
REF VIAIH-502
INTENDED USE
Avian flu is high deadly contagion, with the symptom of acute septic death. The Avian Influenza Virus H5 (AIV H5) Antigen Rapid Test Cassette (Laryngotracheal secretion/Feces) is used for qualitative detection of avian influenza H5 virus in avian trachea and/or feces as an aid in diagnosis of avian influenza H5 virus infection, such as chicken, bird and duck.
PRINCIPLE
The Avian Influenza Virus H5 (AIV H5) Antigen Rapid Test Cassette (Laryngotracheal secretion/Feces) is a qualitative, lateral flow immunoassay for the detection of avian influenza H5 Capsid Protein HA in laryngotracheal secretion or fecal specimens. In this test, antibody specific to the avian influenza H5 Capsid Protein HA is coated on the test line region of the test. During testing, the extracted specimen reacts with the antibody to avian influenza H5 that are coated onto particles. The mixture migrates up the membrane to react with the antibody to avian influenza H5 on the membrane and generate one colored linein the test region. The presence of this colored line in test region indicates a positive result. To serve as a procedural control, a colored line will always appear in the control region if the test has performed properly.
STORAGE AND STABILITY
Store as packaged in the sealed pouch either at room temperature or refrigerated (2-30 °C). The test is stable through the expiration date printed on the sealed pouch. The test cassette must remain in the sealed pouch until use. DO NOT FREEZE. Do not use beyond the expiration date.
PRECAUTIONS
 Do not use after the expiration date. Do not reuse the test kit.
 Handle all specimens as if they contain infectious agents. Observe established precautions against microbiological hazards throughout testing and follow the standard procedures for proper disposal of specimens.
 Wear disposable gloves and eye protection when specimens are being tested.
 Humidity and temperature can adversely affect results.
 Do not remove test cassette from its pouch until immediately before use.
 Do not mix components from different lot and different products.
MATERIALS
Materials Provided
• Test cassettes
• Droppers
• Plastic tubes with buffer
• Swabs
• Package insert
Materials Required but Not Provided
•Timer
DIRECTIONS FOR USE
Allow the test, specimen, buffer and/or controls to equilibrate to room temperature (15-30 °C) prior to testing.
1. Collect fresh poultry laryngotracheal secretions or feces sample approximately mwith the swabs provided.
For laryngotracheal secretions sample, make the swab wet sufficiently.
For fecal sample, the amount of fecal swab as follows:
Note: sample collection directly affects the correctness of test results, so please ensure proper collection amount.
2. Insert the wet swab into the provided plastic tube with buffer. Rotate the swab for approximately 10 seconds while pressing the head against the inside of the tube to release the antigen in the swab. Remove the swab while squeezing the swab head against the inside of the tube as you remove it to expel as much liquid as possible from the swab. Discard the swab in accordance with your biohazard waste disposal protocol.
3. Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer 3 drops of extracted sample (approximately 120 μL) to the specimen well (S) of the test cassette, and then start the timer. See the
illustration below.
4. Read the result at 15 minutes. Do not interpret results after 20 minutes.

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